20.109(F07): ECD assembly

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20.109(F07): Laboratory Fundamentals of Biological Engineering

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Introduction

Protocol

Part 1: Phage titers for M13.1 and MDS

Record the number of plaques for each plate you prepared last time, and then use the most "countable" to calculate PFU/ml for the M13K07/M13.1 and WT/MDS pairs. To collect the class's data in one place, you should then copy two tables to the data collection page.

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6
10-8
10-10

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Part 2: Li overlay

DONE!

For next time

  1. titer?, summary paragraph
  2. summary of ECD, variables and effects
  3. final presentation directions

Reagents

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