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840:153g: Recombinant DNA Te(a)chniques

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Course overview

This page is the homepage of the course titled "840:153g". Current version: Fall 2008!

We have 16 students in class which will form groups of two throughout the semester. Each group will clone a hop (this is the name of the plant :-) gene, put it into E. coli and verify that they cloned a functional gene. Students have to select a gene from the NCBI database and clone the coding region only (no introns are allowed). They need to develop a strategy for cloning, including a procedure for verification that they actually cloned the full coding region of the gene. The approach must be written up in a research proposal and approved by the instructor before lab work can commence. Each group of students will need to develop a website at OWW where they describe their project (in form of the proposal). I require that students write a DAILY online lab notebook for each lab session. This online notebook must contain all procedures carried out during the lab session, including description of results, and discussion of the next steps. Everything must relate to the proposal.

Grading

A large part of the grading will be based on the notebook. It will be graded based on completeness, accuracy, and content. The online notebook will substitute for a paper lab notebook, although I strongly recommend to keep notes during the session to write down observations, calculations, remarks, etc. The notebook has to be written at the day of the lab! No late entries (even 1 day) will be considered. Since each entry in OWW contains a time stamp and the name of user who writes it, this is very easy to monitor. Notebook writing is considered to be part of the work and therefore this work should be done during the lab. The computer room is next door - so please start writing during experimental lag times.

Finally, I expect that each group clones their gene. If a group does not achieve it, the problems must be explained thoroughly in the notebook and evidence must be provided from the notebook that sufficient efforts have been undertaken to clone the gene (after all this is real science and there is no guarantee that all groups will succeed). The notebook will account for 60% of the grade (both partners receive the same grade for the notebook, weighed by a peer assessment). 25% of the grade will assigned by the instructor for contributions during class (including preparation for class, discussion of experiments and results, timeliness, and teamwork). The remaining 15% will be given for the project presentation. One member of each team will give a midterm presentation (before spring break), the other member will give a final presentation (the week before finals).

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