840:153g:Projects/project22/2012/11/01

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Genomic DNA Extraction Attempt 2

  • On Tuesday we began another genomic DNA Extraction of S. marcescens (a culture was grown up from a streak plate on Monday) using protocols from openwetware.org ([[1]]). Today we ran simple gel electrophoresis on our 6 DNA samples. Unfortunately, we had no visible bands displayed.

We suspect this may be because our DNA was not fully resuspended in tris buffer (there was still visible product in the tubes). On Tuesday, we will check our samples to see if there is still visible product in them and if so, we will centrifuge the tubes, pour off the supernatant into another tube and try to suspend the visible product in more tris buffer (this is in case we have too much DNA product in too little buffer). We will then run gel electrophoresis on all the samples.


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