840:153g:Projects/project22/2012/10/25
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Prodigiosin Production in E.Coli | Main project page Previous entry Next entry |
Attempted PCR and Gel Electrophoresis Verification
Lane 3 marker on the right to Lane 12 marker on the left As you can see in the above image, our PCR products (the lanes between the markers) did not show up (even the positive control). This is most likely due to our erroneous PCR cycling. We could confirm the negative results of today's electrophoresis by running another PCR and gel electrophoresis check, but we have had poor results (gel electrophoresis checks with questionable DNA content - I.E. results indistinguishable from random proteins) with this DNA since day 1, so we will start preparing for another DNA extraction next week.
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