840:153g:Projects/project23/2012/11/01: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of the OmcF gene</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
==Continuation of Mutagenesis Reaction  ==
* Insert content here...
* On Tuesday, repeated the PCR preparation that we completed last Thursday. The reason for repeating this experiment was to be able to cut out the bands that contained our supposed gene. Another reason is to be able to clearly compare the bands side by side, which wasn't clear the first time we preformed the PCR and gel electrophoresis.
*On Thursday we prepared two gels, one at 1.0% and the other at 1.8%. We ran the only the replicated primered DNA samples in the 1.0% gel for further extraction. Everything else was run on the 1.8% gel for higher resolution. We ran them overnight at 10V in order to create clearer bands. 




Latest revision as of 22:11, 26 September 2017

Cloning of the OmcF gene Main project page
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Continuation of Mutagenesis Reaction

  • On Tuesday, repeated the PCR preparation that we completed last Thursday. The reason for repeating this experiment was to be able to cut out the bands that contained our supposed gene. Another reason is to be able to clearly compare the bands side by side, which wasn't clear the first time we preformed the PCR and gel electrophoresis.
  • On Thursday we prepared two gels, one at 1.0% and the other at 1.8%. We ran the only the replicated primered DNA samples in the 1.0% gel for further extraction. Everything else was run on the 1.8% gel for higher resolution. We ran them overnight at 10V in order to create clearer bands.


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