840:153g:Projects/project24/2012/10/25: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2012/10/25 Entry for 840:153g:Projects/project24) |
|||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Agarose gel electrophorosis for extraced DNA sample== | ||
| Line 15: | Line 15: | ||
__NOTOC__ | __NOTOC__ | ||
[[category:OWWLabNotebookV1]] | [[category:OWWLabNotebookV1]] | ||
On Tuesday, we completed DNA extraction process. We also ran 0.8% Agarose gel to quantify the DNA that we extracted. We ran 12 samples ( 6 undigested and 6 EcoRI digested) on the gel. Due to some technical problem we couldn't see the bands under UV the same day. So, we rapped our gel into a air tight plastic bag and stored it at 4 degree C. On Wednesday, morning we saw the gel under UV. Out of 12 6 samples gave good bands.DNA 3 undigested didn't showed any bands however, its digested sample showed good bands. So, we decided to run Sample 3 into the gel one more time in our next lab. Also, we decided to mix sample DNA 1 and DNA 5 because they both gave similar intensities. | |||
On Thursday,we had to attend a workshop so we didn't do any lab. | |||
Revision as of 17:26, 25 October 2012
 Project Name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Agarose gel electrophorosis for extraced DNA sample | |
On Tuesday, we completed DNA extraction process. We also ran 0.8% Agarose gel to quantify the DNA that we extracted. We ran 12 samples ( 6 undigested and 6 EcoRI digested) on the gel. Due to some technical problem we couldn't see the bands under UV the same day. So, we rapped our gel into a air tight plastic bag and stored it at 4 degree C. On Wednesday, morning we saw the gel under UV. Out of 12 6 samples gave good bands.DNA 3 undigested didn't showed any bands however, its digested sample showed good bands. So, we decided to run Sample 3 into the gel one more time in our next lab. Also, we decided to mix sample DNA 1 and DNA 5 because they both gave similar intensities.
On Thursday,we had to attend a workshop so we didn't do any lab.
