840:153g:Projects/project4/2009/03/12: Difference between revisions

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*The following primers will be used to clone the '''entire''' protease gene
*The following primers will be used to clone the '''entire''' protease gene


    Forward Primer:
Forward Primer:
         5’ [ATGAATTCGCGGCCGCTTCTAG]ATGGGTTTAGGTAAGAAATT
         5’ [ATGAATTCGCGGCCGCTTCTAG]ATGGGTTTAGGTAAGAAATT


Revision as of 13:02, 12 March 2009

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Thursday 3/12

Oggie

  • Designed primers for neutral protease gene of B. subtilis
  • Gene will be used to test the extracted DNA
  • The following primers will be used to clone the entire protease gene

Forward Primer: 

        5’ [ATGAATTCGCGGCCGCTTCTAG]ATGGGTTTAGGTAAGAAATT

   Reverse Primer:
        5’ [ATCTGCAGCGGCCGCTACTAGTA]TTACAATCCAACAGCATTCCA
  • The following primers will be used to clone just the mature product of the protease gene

Forward Primer: 

        5’ [ATGAATTCGCGGCCGCTTCTAG]ATGGCCGCCGCCACTGGAAG

   Reverse Primer:
        5’ [ATCTGCAGCGGCCGCTACTAGTA]TTACAATCCAACAGCATTCCA


/

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