840:153g:Projects/project4/2009/03/12: Difference between revisions

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==Thursday 3/12==
==Thursday 3/12==
''Oggie''
''Oggie''
*Designed primers for neutral protease gene of ''B. subtilis''
*Designed primers for neutral protease gene of ''B. subtilis'' strain 168
*Gene will be used to test the extracted DNA's and as a backup if aprE can't be cloned again
*Gene will be used to test the extracted DNA's and as a backup if aprE (alkaline protease subtilisin) can't be cloned again
*The following primers will be used to clone the '''entire''' protease gene
*The following primers will be used to clone the '''entire''' protease gene


Revision as of 13:56, 12 March 2009

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Thursday 3/12

Oggie

  • Designed primers for neutral protease gene of B. subtilis strain 168
  • Gene will be used to test the extracted DNA's and as a backup if aprE (alkaline protease subtilisin) can't be cloned again
  • The following primers will be used to clone the entire protease gene
   Forward Primer:
        5’ [ATGAATTCGCGGCCGCTTCTAG]ATGGGTTTAGGTAAGAAATT

   Reverse Primer:
        5’ [ATCTGCAGCGGCCGCTACTAGTA]TTACAATCCAACAGCATTCCA
  • The following primers will be used to clone just the mature product of the protease gene
   Forward Primer:
        5’ [ATGAATTCGCGGCCGCTTCTAG]ATGGCCGCCGCCACTGGAA

   Reverse Primer:
        5’ [ATCTGCAGCGGCCGCTACTAGTA]TTACAATCCAACAGCATTCCA


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