ATc
From OpenWetWare
(Difference between revisions)
(→Preparing working concentration) |
|||
| Line 1: | Line 1: | ||
| - | anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. | + | anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay. |
==Preparing working concentration== | ==Preparing working concentration== | ||
Revision as of 16:27, 1 March 2009
anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.
Preparing working concentration
According to Nallamsetty and Waugh (2007):
Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.
