Altman:Protocols/Preparing Reagents/Making assay buffer: Difference between revisions
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==Making Assay Buffer== | |||
Prepare the following beforehand: | |||
*100x DTT stock: 100 mM dithiothreitol | |||
*10x BSA stock: 10 mg/mL bovine serum albumin | |||
*10x AB stock: 250 mM KCl, 250 mM Imidazole-Cl (pH 7.4), 10 mM EGTA, 40 mM MgCl<sub>2</sub> | |||
<table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | <table id="Table 1" border="1" cellpadding="3" cellspacing="0"> | ||
<tr> | <tr> | ||
<th><tt> </tt></th> | <th><tt> </tt></th> | ||
<th><tt>1 mL Assay Buffer</tt></th> | <th><tt>1 mL Assay Buffer | ||
<th><tt>1 mL Assay Buffer + BSA</tt></th> | |||
(AB) | |||
</tt></th> | |||
<th><tt>1 mL Assay Buffer + BSA | |||
(ABSA) | |||
</tt></th> | |||
</tr> | </tr> | ||
Latest revision as of 18:43, 10 November 2012
Department of Physics, Willamette University
Making Assay Buffer
Prepare the following beforehand:
- 100x DTT stock: 100 mM dithiothreitol
- 10x BSA stock: 10 mg/mL bovine serum albumin
- 10x AB stock: 250 mM KCl, 250 mM Imidazole-Cl (pH 7.4), 10 mM EGTA, 40 mM MgCl2
| 1 mL Assay Buffer
(AB) |
1 mL Assay Buffer + BSA
(ABSA) |
|
|---|---|---|
| 10x AB stock | 100 uL | 100 uL |
| 100x DTT stock | 10 uL | 10 uL |
| 10x BSA stock | -- | 100 uL |
| ddwater | 890 uL | 790 uL |
