Altman:Protocols/TC Protocols/Thawing ARPE-19

From OpenWetWare
Revision as of 15:02, 4 September 2014 by David Altman (talk | contribs) (→‎Splitting ARPE-19 cells)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search


Department of Physics, Willamette University

'


Home        Contact        Lab Members        Publications        Research        Protocols        Pictures       


Thawing ARPE-19 cells

Before starting:

  • Warm up Media+Serum in 37°C water-bath
  • Place a beaker for disposing of waste in the hood
  • Place a T-25 Flask in the hood
  • Place 9 mL of warmed Media+Serum into a 15 mL conical


1. Thaw aliquot of cells in gloved hands

2. Move cells into the 9 mL of media

3. Spin cells at 500 rpm for 3 minutes

4. Remove supernatant

5. Re-suspend the pellet in 5 mL of media

6. Place the cells into a T-25 flask

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Altman:Protocols/TC_Protocols/Thawing_ARPE-19&oldid=816254"