Antibodies

Drawing of an antibody - heavy chains red, light chains yellow.

General

• Griffin:Antibody Basics - Korey's introduction to antibodies
• Antibody entry in the Wikipedia

Antibody protocols

Korey's treasure trove of antibody-related protocols

• Antibody Basics
• Antibody Peptide Neutralization
• Antibody Protein Coupling (F:P) Ratios
• Antibody Elution Buffers
• Antibody Related Solutions & Recipes
• Antigen Retrieval Technique
• Chromatin Immunoprecipitation
• Dual Immunofluorescence Cell Staining
• Flow Cytometry prep & labeling
• Immunoprecipitation Guide
• Immunohistochemistry Paraffin
• Immunofluorescence Cell Staining
• Membrane Stripping and Reprobing
• Western Blotting Guide
• Western Blot Optimization

General protocols & pages involving antibodies

• Generating antibodies
• Protein blot (Western)
• Dot Blot
• Affinity purification of antibodies

Lab-specific antibody protocols

• Hoatlin:Crosslinking antibodies to beads
• Sauer:Western blot
• Silver:Immunofluorescence
• Wittrup: Protein conjugation with fluorophores
• Silver: FISH/IF
• Berglund:Antibody Storage

Other related pages

• Antibodypedia
• Toolbox lecture including ELISA