Antibody:X
 | |
|---|---|
| |
| Cross-Reactive w | human |
| Not Cross-Reactive w | mouse, rat |
| Raised Against | peptide |
| Suitability for.. | |
| Western Blot | worked |
| Immunoprecipitation | untested |
| Staining fixed cells | uncertain |
| Staining cryo-sections | worked |
| Staining paraffin sections | failed |
| Help | |
Suggested changes to infobox template
Header
I would favour anti Tubulin instead of the current Name: Tubulin. It's a frequent short hand for antibody against tubulin. The Greek letter looks strange in this font.
Uniprot links
Is it possible to generate a link from the UniProt ID similar to PMID 1234567? Something like Uniprot P23258. As far as I understand UniProt currently has the following URL structure: http://www.uniprot.org/uniprot/P23258
Additional field
I'm wondering whether antibody provider should be an infobox field. Nowadays, most antibody are purchased from companies instead of generated in the lab. In other words, most antibodies will have 1+ commercial provider that could be linked to here or in the main page.
Putting parameters in order
Suggested re-ordering of parameters:
- Target - most important info; should come top
- Antibody type
- Cross-reactive (with)
- Not cross-reactive (with)
Regarding experiment paraffin is generally the most challenging technique for an antibody, so it should come last after cryo.
Subdividing infobox parameters
Logically, the bottom 5 are separate from the top half - experiments vs. antibody properties. It would be great if this were highlighted graphically, e.g. by drawing an additional box around the top and bottom halves. The bottom half could have the heading: "Suitability for.." or sth similar.
Antibody type
- we can either use description to contain source organisms and antibody type (e.g. mouse monoclonal IgG); antibody type would be more descriptive than description
- OR
- split it into 2 fields:
- clonality: monoclonal or polyclonal (currently only antibodies raised in mice can be monoclonal, although that might change as technology advances)
- raised in species: mouse, rat, guinea pig, rabbit, goat, chicken,..
- Need input: you tell me. Will do as you want. - let's go for one field since currently all non-mouse Ab are polyclonal
Past changes
Parameters
- target: e.g. standard protein name, possibly link to UniProt or NCBI protein
- OK - put in an example target protein; abbreviation in target field, full name in box header
- raised against: full-length protein, peptide
- OK
- cross-reactive against: mouse, rat, human,.. (this will always be the species it was raised against, here human and may be more species)
- OK
- not cross-reactive against: as above
- OK - made this italic to offset it from the species where this antibody can be used
For documentation
Usability for experiments
Types of experiments:
- Protein blot (Western)
- Immunoprecipitation (IP)
- Staining fixed cells (ICC)
- Staining paraffin sections (IHC-paraffin)
- Staining cryo-sections (IHC-cryo)
- (I replaced the more cryptic names immunohistochemistry.. with more descriptive and more easily understood names)
- OK
Usability evaluation:
- worked
- uncertain
- failed
- untested
- OK Coloring is automatic if one of the 4 tags above used. Otherwise it will be black. - Great, I like that.
Description in main page
- antibody created by..
Experimental evidence for infobox claims
Images
Description, conclusions..
Providers
- company A with link & possible price
- company B with link
Related Antibodies
List all related antibodies here.
