BCH4160/2011:Notebook/Laura Lee/2011/10/11: Difference between revisions
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Refer to "Crime Scene Investigator: PCR Basics™ Kit". Biotechnology Explorer™. Bio--Rad Laboratories, Inc. for the complete procedure. | Refer to "Crime Scene Investigator: PCR Basics™ Kit". Biotechnology Explorer™. Bio--Rad Laboratories, Inc. for the complete procedure. | ||
==References== | ==References== | ||
Revision as of 07:45, 18 November 2011
PCR continued
Refer to part 1 for purpose of experiment.
Materials
Refer to part 1 for initial materials. Refer to "Crime Scene Investigator: PCR Basics™ Kit". Biotechnology Explorer™. Bio--Rad Laboratories, Inc. for complete list of materials.
Gel electrophoresis/Staining of Gel
- Gel electrophoresis system
- Previously amplified DNA samples (from part 1)
- Agarose
- 50x TAE buffer
- Fast Blast™ DNA Stain
Procedure
The purpose of this part of the procedure was to use gel electrophoresis to separate and visualize the DNA fragments.
Refer to "Crime Scene Investigator: PCR Basics™ Kit". Biotechnology Explorer™. Bio--Rad Laboratories, Inc. for the complete procedure.
References
Relevant papers and books
- Goldbeter, A and Koshland, DE (1981) - Proc Natl Acad Sci U S A 78(11) 6840-4 PMID 6947258
- Jacob, F and Monod, J J (1961) - Mol Biol 3(3) 318-56 PMID 13718526
- Ptashne, M (2004) Genetic Switch: Phage Lambda Revisited - Cold Spring Harbor Laboratory Press ISBN 0879697164
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
