BCH4160/2011:Notebook/Laura Lee/2011/10/11: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 25: Line 25:




==Contact==
==Results==
*Who has experience with this protocol?


or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].  
No results were gained from this experiments. Errors were made in gel staining (overstaining) and bands were not visually distinguishable.
 
<!-- You can tag this protocol with various categories. See the [[Categories]] page for more information. -->
 
<!-- Move the relevant categories above this line to tag your protocol with the label
[[Category:Protocol]]
 
[[Category:Needs attention]]
[[Category:In vitro]]
 
[[Category:In vivo]]
 
[[Category:In silico]]
 
[[Category:DNA]]
 
[[Category:RNA]]
 
[[Category:Protein]]
 
[[Category:Chemical]]
 
[[Category:Escherichia coli]]
 
[[Category:Yeast]]
-->

Revision as of 07:47, 18 November 2011

PCR continued

Refer to part 1 for purpose of experiment.

Materials

Refer to part 1 for initial materials. Refer to "Crime Scene Investigator: PCR Basics™ Kit". Biotechnology Explorer™. Bio--Rad Laboratories, Inc. for complete list of materials.

Gel electrophoresis/Staining of Gel

  • Gel electrophoresis system
  • Previously amplified DNA samples (from part 1)
  • Agarose
  • 50x TAE buffer
  • Fast Blast™ DNA Stain

Procedure

The purpose of this part of the procedure was to use gel electrophoresis to separate and visualize the DNA fragments.

Refer to "Crime Scene Investigator: PCR Basics™ Kit". Biotechnology Explorer™. Bio--Rad Laboratories, Inc. for the complete procedure.



Results

No results were gained from this experiments. Errors were made in gel staining (overstaining) and bands were not visually distinguishable.

Retrieved from "https://openwetware.org/mediawiki/index.php?title=BCH4160/2011:Notebook/Laura_Lee/2011/10/11&oldid=567383"