BISC110/F09:Series2: Difference between revisions
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#How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants. | #How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants. | ||
#How your own genotype and phenotype at the PTC locus correlate. | #How your own genotype and phenotype at the PTC locus correlate. | ||
#About the ethical and policy issues which surround the use of SNPs in medicine. | #About the ethical and policy issues which surround the use of SNPs in medicine.<br><br> | ||
The following labs adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor. The American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html). | |||
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[[BISC110: Series 3 Lab 5 Taster SNP1 | Lab 5 Taster SNP 1]]<br> | [[BISC110: Series 3 Lab 5 Taster SNP1 | Lab 5 Taster SNP 1]]<br> |
Revision as of 04:53, 7 August 2009
Series 2- To taste or not to taste
In this three week series of experiments you will learn:
- About SNPs and how they are analyzed.
- How to isolate DNA from your own cells.
- How to use PCR to amplify a region of your PTC gene which contains a SNP.
- How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
- How your own genotype and phenotype at the PTC locus correlate.
- About the ethical and policy issues which surround the use of SNPs in medicine.
The following labs adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor. The American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).