Series 2- To Taste Or Not To Taste
In this three week series of experiments you will learn:
- About SNPs and how they are analyzed.
- How to isolate DNA from your own cells.
- How to use PCR to amplify a region of your PTC gene which contains a SNP.
- How to use restriction enzymes and gel electrophoresis to identify the two most common PTC gene variants.
- How your own genotype and phenotype at the PTC locus correlate.
- About the ethical and policy issues which surround the use of SNPs in medicine.
The following labs were adapted from Merritt, R. B., et. al. 2008. Tasting phenylthiocarbamide (PTC): a new integrative genetics lab with an old flavor and from the American Biology Teacher, 70:23-28 and Human Single Nucleotide Polymorphism Determination, by S. Deel, S. Singer and D. Walser-Kuntz (http://serc.carleton.edu/genomics/units/snp.html).
Lab 5 Taster SNP 1
Lab 6: Taster SNP 2
Lab 7: Taster SNP 3