BISC110/F09:Series3: Difference between revisions

Revision as of 10:42, 8 June 2009

Wellesley College-BISC110 Introduction to Cell Biology-Fall 2009

At the end of this three week series on photosynthesis (Labs 8-10) you will have mastered the following skills and concepts.

In this semester long experiment you will:

@@ Line 8: / Line 8: @@
 '''In this semester long experiment you will:'''   <br>
-#use the Agrobacterium system to introduce a foreign gene, the β -glucuronidase gene (gusA) of E. coli, into the cells of tobacco<br>
+#<br>
-#take advantage of the totipotency of plant cells and use these transformed cells to regenerate genetically engineered plants<br>
+#<br>
-#confirm that the regenerated plants are transformed by<br>
+#<br>
-##measuring the activity of the introduced enzyme, β–glucuronidase (GUS) spectrophotometrically and histochemically
+##
-##PCR to test directly for the presence of the introduced genes and  <br>
+##<br>
-#become adept at sterile technique.
+#
@@ Line 21: / Line 20: @@
 ! Concepts !! Genes/Organisms !! Techniques/Skills
 |-
-| Effect of random gene insertion in a large genome
+|
-| Bacterial pathogen:  ''Agrobacterium tumefaciens'' || Aseptic tissue culture
+|  ||
 |-
-| Plant totipotency
+|
-|Tobacco plant: ''Nicotiana tobacum''
+|
-|DNA Extraction
+|
 |-
-|Regulation of gene expression
+|
-|gusA gene from ''E. coli''
+|
-|PCR
+|
 |-
-|Genetic Engineering and creation of genetically modified organism
 |
-|Agarose gel electrophoresis
+|
+|
 |-
-|Phenotypic Selection
 |
-|Transfection & selection by antibiotic resistance;
+|
-Enzyme function assays: colorimetric & histochemical
+|
 |}