BISC209:Protcols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Microbiology Protocols)
Current revision (14:54, 28 February 2010) (view source)
(Microbiology Protocols)
 
(8 intermediate revisions not shown.)
Line 5: Line 5:
==Microbiology Protocols==
==Microbiology Protocols==
[[BISC209: Agar Overlay | Agar Overlay (Pour) Plates ]]<BR>
[[BISC209: Agar Overlay | Agar Overlay (Pour) Plates ]]<BR>
-
[[BISC209: Aseptic Transfer |  Aseptic Transfer ]]<BR>
+
[[BISC209: Aseptic Transfer |  Aseptic Transfer: Making Slants, Broth to Broth or Plate Transfer ]]<BR>
[[BISC209: Calibration of Micropipets | Calibration of Micropipets ]]<BR>
[[BISC209: Calibration of Micropipets | Calibration of Micropipets ]]<BR>
[[BISC209: Carbohydrate Fermentation Medium|Carbohydrate Fermentation Medium]]<BR>
[[BISC209: Carbohydrate Fermentation Medium|Carbohydrate Fermentation Medium]]<BR>
Line 19: Line 19:
[[BISC209: Simple Stains | Simple Stains]]<BR>
[[BISC209: Simple Stains | Simple Stains]]<BR>
[[BISC209: Preparing a bacterial smear slide | Smear Slide Preparation]]<BR>
[[BISC209: Preparing a bacterial smear slide | Smear Slide Preparation]]<BR>
 +
[[BISC209: Recipes | Recipes for test medium and reagents]]<BR>
 +
 +
[[BISC209: Stock cultures | Stock cultures ]]<BR>
[[BISC209: Stains | Stains (Special): Endospore, Acid fast, Capsule, and Flagella]]<BR>
[[BISC209: Stains | Stains (Special): Endospore, Acid fast, Capsule, and Flagella]]<BR>
[[BISC209: Streaking for Isolation | Streaking for Isolation ]]<BR>
[[BISC209: Streaking for Isolation | Streaking for Isolation ]]<BR>
-
[[BISC209: Streptomyces isolation from soil |Streptomyces isolation from soil]]<BR>
 
[[BISC209: Roles of soil Microbes |Tests to determine the role of a soil isolate]]<BR>
[[BISC209: Roles of soil Microbes |Tests to determine the role of a soil isolate]]<BR>
-
[[BISC209: Selective,and/or Differential, and/or Enriched media |Use of Selective, Differential, Enrichment Media for Identification for Gram-negative Enterobacteriacae]]<BR>
+
[[BISC209: Selective,and/or Differential, and/or Enriched media |Use of Selective, Differential, Enrichment Media]]<BR>
 +
 
 +
[[BISC209: wet mounts | Wet Mount procedure ]]<BR>
==Links to Labs==
==Links to Labs==

Current revision

Wellesley College-BISC 209 Microbiology -Spring 2010

Microbiology Protocols

Agar Overlay (Pour) Plates
Aseptic Transfer: Making Slants, Broth to Broth or Plate Transfer
Calibration of Micropipets
Carbohydrate Fermentation Medium
Enrichment Media for Soil Bacterial from Mixed Populations
Enterotube Protocol
Enzyme tests
Gram Stain
Microscopy: Care and Use of the Compound Brightfield Microscope
Motility Tests
Pouring Media To Make Solid Agar Plates
Rhizobium isolation from soil
Serial Dilution
Simple Stains
Smear Slide Preparation
Recipes for test medium and reagents

Stock cultures
Stains (Special): Endospore, Acid fast, Capsule, and Flagella
Streaking for Isolation
Tests to determine the role of a soil isolate
Use of Selective, Differential, Enrichment Media

Wet Mount procedure

Links to Labs

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11

Lab 12
Personal tools