BISC209: Stock cultures
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Stock Cultures
Lab 1:
Staphylococcus epidermidis
Serratia marcescens maintained at both 27 (red) and 37 (white)
Lab 4:
Gram pos: PEA growth
Staph epi.
Gram neg EMB growth
any of these:
Table 2. Colonial appearance on EMB Agar after 18-24 hours at 35°C. A differential medium can be used to differentiate Gram negative enteric organisms based on the colony color.
Organism | Colonial Appearance |
---|---|
Escherichia coli | purple with black center/ green metallic sheen |
Klebsiella pneumoniae | dark centered colonies/ green metallic sheen |
Enterobacter aeorogenes | pink colonies/ no metallic sheen |
Proteus mirabilis | colorless colonies |
Salmonella typhimurium | colorless colonies |
Lab 5
-
positive control:
Bacillus or Staph. epi
Negative control:
Enterococcus faecalis
- Escherichia coli
-
Eschericia coli (Gram negative), Staphylococcus epidermidis (Gram positive) and Micrococcus luteus
Starch plate-
pos. controls Bacillus subtilis
neg control: Staph. sp.?
Lab 6
MRVP TEST
-
MR pos control: Escherichia coli
VP pos control: Enterobacter aerogenes
Links to Labs
Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12
-
pos. controls Bacillus subtilis