BISC209: Stock cultures: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 48: | Line 48: | ||
Antibiotic plates control orgs (2 Gram pos 1 Gram neg):<UL><LI> | Antibiotic plates control orgs (2 Gram pos 1 Gram neg):<UL><LI> | ||
''Eschericia coli'' (Gram negative), ''Staphylococcus epidermidis'' (Gram positive) and ''Micrococcus luteus'' | ''Eschericia coli'' (Gram negative), ''Staphylococcus epidermidis'' (Gram positive) and ''Micrococcus luteus''<BR><BR | ||
Lab 6 < | |||
Lab 6 <UL><LI> | |||
MR pos control: Escherichia coli<BR> | MR pos control: Escherichia coli<BR> | ||
VP pos | VP pos control: Enterobacter aerogenes</LI></UL> | ||
==Links to Labs== | ==Links to Labs== | ||
Revision as of 12:30, 18 January 2010
Stock Cultures
Lab 1:
Staphylococcus epidermidis
Serratia marcescens maintained at both 27 (red) and 37 (white)
Lab 4:
Gram pos: PEA growth<BR
Staph epi.
Gram neg EMB growth
any of these:
Table 2. Colonial appearance on EMB Agar after 18-24 hours at 35°C. A differential medium can be used to differentiate Gram negative enteric organisms based on the colony color.
Lab 5
catalase test
-
positive control:
Bacillus spp or Staph. epi
Negative control:
Enterococcus faecalis
- Escherichia coli
-
Eschericia coli (Gram negative), Staphylococcus epidermidis (Gram positive) and Micrococcus luteus
<BR Lab 6-
MR pos control: Escherichia coli
VP pos control: Enterobacter aerogenes
Links to Labs
Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12
-
MR pos control: Escherichia coli
| Organism | Colonial Appearance |
|---|---|
| Escherichia coli | purple with black center/ green metallic sheen |
| Klebsiella pneumoniae | dark centered colonies/ green metallic sheen |
| Enterobacter aeorogenes | pink colonies/ no metallic sheen |
| Proteus mirabilis | colorless colonies |
| Salmonella typhimurium | colorless colonies |
