BISC209: Stock cultures: Difference between revisions
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==Stock Cultures== | |||
'''Lab 1:'''<BR> | |||
''Staphylococcus epidermidis''<BR> | |||
''Serratia marcescens'' maintained at both 27 (red) and 37 (white)<BR> | |||
'''Lab 4:''' <BR> | |||
Gram pos: PEA growth<BR> | |||
''Staph epi.''<BR><BR> | |||
Gram neg EMB growth <BR> | |||
any of these:<BR> | |||
'''Table 2.''' Colonial appearance on EMB Agar after 18-24 hours at 35°C. A differential medium can be used to differentiate Gram negative enteric organisms based on the colony color.<BR> | |||
{| border="1" | |||
|+ | |||
! '''Organism''' !! '''Colonial Appearance''' | |||
|- | |||
! ''Escherichia coli'' | |||
| purple with black center/ green metallic sheen | |||
|- | |||
! ''Klebsiella pneumoniae'' | |||
| dark centered colonies/ green metallic sheen | |||
|- | |||
! ''Enterobacter aeorogenes'' | |||
| pink colonies/ no metallic sheen | |||
|- | |||
! ''Proteus mirabilis'' | |||
| colorless colonies | |||
|- | |||
! ''Salmonella typhimurium'' | |||
| colorless colonies | |||
|- | |||
|} | |||
<BR><BR> | |||
'''Lab 5'''<BR> | |||
catalase test<UL><LI> | |||
positive control: <BR> | |||
''Bacillus'' or ''Staph. epi''<BR><BR> | |||
Negative control:<BR> | |||
''Enterococcus faecalis''<BR> | |||
</LI></UL> | |||
oxidase test<UL><LI> | |||
''Escherichia coli''</LI></UL> | |||
Antibiotic plates control orgs (2 Gram pos 1 Gram neg):<UL><LI> | |||
''Eschericia coli'' (Gram negative), ''Staphylococcus epidermidis'' (Gram positive) and ''Micrococcus luteus''<BR><BR> | |||
Starch plate <UL><LI> | |||
pos. controls ''Bacillus subtilis'' <BR> | |||
neg control: ''Staph. sp.''?<BR> | |||
</LI></UL> | |||
'''Lab 6 '''<BR> | |||
MRVP TEST <UL><LI> | |||
MR pos control: ''Escherichia coli''<BR> | |||
VP pos control: ''Enterobacter aerogenes'' | |||
</LI></UL> | |||
==Links to Labs== | ==Links to Labs== |
Latest revision as of 19:16, 19 January 2010
Stock Cultures
Lab 1:
Staphylococcus epidermidis
Serratia marcescens maintained at both 27 (red) and 37 (white)
Lab 4:
Gram pos: PEA growth
Staph epi.
Gram neg EMB growth
any of these:
Table 2. Colonial appearance on EMB Agar after 18-24 hours at 35°C. A differential medium can be used to differentiate Gram negative enteric organisms based on the colony color.
Organism | Colonial Appearance |
---|---|
Escherichia coli | purple with black center/ green metallic sheen |
Klebsiella pneumoniae | dark centered colonies/ green metallic sheen |
Enterobacter aeorogenes | pink colonies/ no metallic sheen |
Proteus mirabilis | colorless colonies |
Salmonella typhimurium | colorless colonies |
Lab 5
-
positive control:
Bacillus or Staph. epi
Negative control:
Enterococcus faecalis
- Escherichia coli
-
Eschericia coli (Gram negative), Staphylococcus epidermidis (Gram positive) and Micrococcus luteus
Starch plate-
pos. controls Bacillus subtilis
neg control: Staph. sp.?
Lab 6
MRVP TEST
-
MR pos control: Escherichia coli
VP pos control: Enterobacter aerogenes
Links to Labs
Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
Lab11
Lab 12
-
pos. controls Bacillus subtilis