BM20: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: '''BM20 media''' 10% low endotoxin FCS 20% L-cell supernatant 5% horse serum 1% glutamine 1% sodium pyruvate '''no Abx''' to final volume with low endo DMEM 630ml low endotoxin DMEM 100m...) |
No edit summary |
||
| Line 1: | Line 1: | ||
'''BM20 media''' | '''BM20 media''' | ||
10% low endotoxin FCS | *10% low endotoxin FCS | ||
20% L-cell supernatant | *20% L-cell supernatant | ||
5% horse serum | *5% horse serum | ||
1% glutamine | *1% glutamine | ||
1% sodium pyruvate | *1% sodium pyruvate | ||
'''no Abx''' | *'''no Abx''' | ||
to final volume with low endo DMEM | *to final volume with low endo DMEM | ||
630ml low endotoxin DMEM | '''1L''' | ||
100ml low endotoxin FCS | *630ml low endotoxin DMEM | ||
200ml L-cell supernatant | *100ml low endotoxin FCS | ||
*200ml L-cell supernatant | |||
* 50ml horse serum | |||
* 10ml glutamine | |||
* 10ml sodium pyruvate | |||
* '''No Abx''' | |||
Latest revision as of 13:19, 6 June 2007
BM20 media
- 10% low endotoxin FCS
- 20% L-cell supernatant
- 5% horse serum
- 1% glutamine
- 1% sodium pyruvate
- no Abx
- to final volume with low endo DMEM
1L
- 630ml low endotoxin DMEM
- 100ml low endotoxin FCS
- 200ml L-cell supernatant
- 50ml horse serum
- 10ml glutamine
- 10ml sodium pyruvate
- No Abx
