BM20

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BM20 media

  • 10% low endotoxin FCS
  • 20% L-cell supernatant
  • 5% horse serum
  • 1% glutamine
  • 1% sodium pyruvate
  • no Abx
  • to final volume with low endo DMEM

1L

  • 630ml low endotoxin DMEM
  • 100ml low endotoxin FCS
  • 200ml L-cell supernatant
  • 50ml horse serum
  • 10ml glutamine
  • 10ml sodium pyruvate
  • No Abx
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