BMCB625:DNA Replication (New components): Difference between revisions
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Numerous studies have suggested that the Mcm2-7 complex does not display helicase activity in vitro, however individual subcomplexes (namely Mcm-4, -6, & -7) do display helicase activity. | |||
Also, Kearsy and Labib (1998) propesed that MCM2-7 proteins bind to chromatin in a cell-cycle dependent manner (being tightly bound in late mitosis and G1; being removed in S- and G2-phase) (Maiorano et al, Kearsy and Labib). | |||
Why might these features be beneficial during replication of chromosomes in eukaryotes? Hint: what is the proposed function of this entire complex in the Moyer paper. | |||
--[[User:Chris|Chris]] 12:47, 17 April 2007 (EDT) | |||
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Revision as of 09:47, 17 April 2007
(Homework) Questions
Jon
Chris
Numerous studies have suggested that the Mcm2-7 complex does not display helicase activity in vitro, however individual subcomplexes (namely Mcm-4, -6, & -7) do display helicase activity.
Also, Kearsy and Labib (1998) propesed that MCM2-7 proteins bind to chromatin in a cell-cycle dependent manner (being tightly bound in late mitosis and G1; being removed in S- and G2-phase) (Maiorano et al, Kearsy and Labib).
Why might these features be beneficial during replication of chromosomes in eukaryotes? Hint: what is the proposed function of this entire complex in the Moyer paper.
--Chris 12:47, 17 April 2007 (EDT)
Mahta
- What do you think is the best way to determine the role of post-translational modifications in the formation of the CMG complex / activation of the helicase?
