BMCB625:Noncoding Y RNA
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(Homework) Questions
Larry
Chayne
Jon
Chris
There appears to be a bulge in the stem structure of Y-RNA’s similar to that present in IRE’s (Iron Responsive Elements); in IRE’s this “stem-bulge” is critical for recognition by the Iron-Responsive Protein (IRP).
Does the bulge on the stem structure serve a specific a function in Y-RNA’s? (First off, is it real, and conserved? The "CG-bulge" is most froquently mutated in psuedogene Y-RNA (Perreault, et al., Nucleic Acids Res. 2005; 33(6): 2032–2041).
Structurally, this could be tested with a variety of Nuclease treatments. e.g., RNase V1 (dsRNA), RNase T1 (ssG), RNaseA (ssU & ssC)) Any other ideas for testing experimentally?
