BME100 f2013:W1200 Group15 L4: Difference between revisions
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'''DNA Sample Set-up Procedure''' | '''DNA Sample Set-up Procedure''' | ||
1. Receive 8 sample tubes from Professor, containing 50μL of PCR reaction material<br> | |||
2. Label Samples according to table, to avoid swapping results<br> | |||
3. Put appropriate DNA sample into the accordingly labeled tube. Use a new pipette tip for each test tube to avoid cross contamination<br> | |||
4. Place the 8 sample tubes into the Thermocycler<br> | |||
5. Follow Thermocycler instructions above<br> | |||
'''PCR Reaction Mix''' | '''PCR Reaction Mix''' | ||
1. Taq DNA polymerase <br> | |||
2. MgCl2 <br> | |||
3. dNTP's <br> | |||
'''DNA/ primer mix''' | '''DNA/ primer mix''' | ||
1. Sample of patient's DNA <br> | |||
2. Forward Primer <br> | |||
3. Reverse Primer <br> | |||
Revision as of 12:59, 23 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsThermal Cycler Program
DNA/ primer mix
1. Sample of patient's DNA
Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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