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The Original Design
(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)
Experimenting With the Connections
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)
The LCD screen turned off.
When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
The temperature changed from 26.7 celsius degree to -40 celsius degree.
Test Run
(Write the date you first tested Open PCR and your experience(s) with the machine)
The Open PCR was first tested on October 10th.
Protocols
Thermal Cycler Program
DNA Sample Set-up
Tube A: Positive Control: cancer DNA template
Tube B: Patient 1 ID-16946 Replicate 1
Tube C: Patient 1 ID-16946 Replicate 2
Tube D: Patient 1 ID-16946 Replicate 3
Tube E: Negative Control: non-cancer DNA template
Tube F: Patient 2 ID-46296 Replicate 1
Tube G: Patient 2 ID-46296 Replicate 2
Tube G: Patient 2 ID-46296 Replicate 3
DNA Sample Set-up Procedure
Step 1: Fill each reaction tube with 50μL of the PCR reaction mix and label all tubes according to the chart above
Step 2: Add 50μL of DNA sample mix to the corresponding reaction tube (Note: use each disposable pipette tip only once to avoid cross-contamination)
Step 3: Place to reaction tubes into the thermocycler and run the above program
PCR Reaction Mix
The PCR reaction mix will contain 8 tubes. Each tube will contain 50μL of the following:
Taq DNA polymerase
MgCl2
dNTP's
DNA/ primer mix
What is in the DNA/ primer mix?
Research and Development
PCR - The Underlying Technology
(Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet)
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)