BME100 f2013:W900 Group10 L5: Difference between revisions
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'''Solutions Used for Calibration''' ''[Instructions: See worksheet page 6.]'' | '''Solutions Used for Calibration''' ''[Instructions: See worksheet page 6.]'' | ||
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Revision as of 20:16, 6 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye SBYR green dye is a cyanine dye used as a nucleic acid stain. It binds to DNA which absorbs of blue light and emits green light. The stain binds to the double stranded DNA and stain the single stranded DNA with lower performance. It can also stain RNA with lower performance. It is also the most sensitive stain available for detecting double stranded DNA during PCR.
Device used to measure parameter of fluorescence such as intensity and wavelength and the display of emission spectrum after excitation by a certain spectrum of light. It detects and measures ultraviolet radiation by determining the amount of fluorescence that it produces from a phosphor fluorometric.
[Instructions: A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
A droplet is placed on the hydrophobic slide which allows the droplet to hold its spherical shape. Then the slides are exposed to two light beams from either sides which decreases the surrounding noise. The upper beam passes through a filter that passes through a sample. The lower beam is passed through an attenuator and tries to match the fluorescent power given off from the sample. Both light is detected by separate transducers and turned into an electrical signal.
ProcedureSmart Phone Camera Settings
[Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part]
[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[Instructions: See worksheet page 6.]
Data AnalysisRepresentative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]
[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]
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