BME100 f2013:W900 Group12 L4
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design This is an image of a device called an Open PCR machine. An Open PCR Machine is a device that performs polymerase chain reactions in which DNA is modified by creating an environment that provides necessary temperature changes for this process to occur. The temperature changes consist of being heated and cooled for multiple cycles; the DNA present within the samples is separated when heated and then replicated when cooled, which in turn results in multiple copies from a single strand of DNA. The Open PCR machine can then be connected to the computer using the USB cable, which enables the user to control the number of cycles and temperatures for the experiment. With this information, the Open PCR Machine then will perform environmental changes on the DNA samples with the heating plate, heating lid, and cooling fan. Information is then processed by the circuit board, and transferred to the LED screen which displays the results.
Experimenting With the Connections When we unplugged (part 3) from (part 6), the LCD display on the machine turned off and did not display anything. When we unplugged the white wire that connects (part 6) to (part 2), the machine was unable to take temperature readings.
ProtocolsThermal Cycler Program Stage 1: 95°C for 3 minutes-DNA double helix unravels Stage 2: Denature; 95°C for 30 seconds-Two DNA strands separate further into two more DNA strands Anneal; 57°C for 30 seconds-Primer attaches to specific DNA sequence Extend; 72°C for 30 seconds-DNA polymerase attaches to primer and begins to add complementary nucleotides to template DNA and extend the chain Stage 3: 72°C for 3 minutes-Polymerase extends the nucleotide chain to the complementary template DNA. Target sequence begins to form. Stage 4: Final Hold; 4°C-Allows for short term storage of the reaction by stopping the thermal cycling process>
PCR Reaction Mix
- Tag DNA polymerase - MgCl2 - dNTP's
+ Reverse Primer Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragraphs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
|