BME100 f2013:W900 Group14 L4: Difference between revisions
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THE COMPONENTS OF PCR<br> | THE COMPONENTS OF PCR<br> | ||
There are three steps in PCR, these steps are: 1)Denaturation, 2)Annealing, and 3)Extension. | There are three steps in PCR, these steps are: 1)Denaturation, 2)Annealing, and 3)Extension. | ||
During the Denaturation, the DNA samples are heated to slightly under the boiling point of water, or around 94 degrees C. At this point the DNA strands break apart at the hydrogen bonds between the complementary bases. During the annealing step, the DNA sample is cooled to around 54 degrees C where the forward and reverse primers attach on the single stranded DNA as the polymerase binds them. | During the Denaturation, the DNA samples are heated to slightly under the boiling point of water, or around 94 degrees C. At this point the DNA strands break apart at the hydrogen bonds between the complementary bases, into the two basic strands that compose non-denatured DNA. During the annealing step, the DNA sample is cooled to around 54 degrees C where the forward and reverse primers attach on the single stranded DNA as the polymerase binds them, so as to allow the DNA Taq Polymerase to bind to the strands and create complementary copies. Finally, during the extension phase, the DNA is reheated to 72 degrees Farenheit, so as to stimulate the polymerase base pair copying reaction to build new DNA. | ||
Latest revision as of 08:59, 30 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine's screen went black. The LCD screen did not receive power while the rest of the machine still did. When we unplugged the white wire that connects (part 6) to (part 2), the machine did not read temperature properly. It briefly fluctuating upon detaching the wire, then read -40 degrees C.
Wednesday, October 23, 2013
ProtocolsThermal Cycler Program
PCR Reaction Mix
DNA/ primer mix
Research and DevelopmentPCR - The Underlying Technology THE COMPONENTS OF PCR
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