BME100 f2013:W900 Group15 L5

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Saiswathi Javangula Name: Justin Dombrowski Name: abdulrahman alruwaythi Name: Ryan Fisher Name: Gage Bebak Name: student LAB 5 WRITE-UP Background Information SYBR Green Dye SYBR green dye is used to stain and dye the nucleic acid which makes it possible to see large amounts of DNA by the naked eye. Single-Drop Fluorimeter The single drop fluorimeter is the device that shines blue light through a drop of DNA/SYBR green dye mix which absorbs the blue light making it possible to see the DNA in the drop of liquid. How the Fluorescence Technique Works the fluorescent technique works because it gives the person the ability to see DNA with the naked eye so that the proof of DNA is apparent Procedure Smart Phone Camera Settings [Instructions: The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Type of Smartphone: iPhone Flash: Flash was inactivated. ISO setting: ISO was set at 800. White Balance: White Balance was set at auto. Exposure: Exposure was set to the highest setting. Saturation: Saturation was set to the highest setting. Contrast: Contrast was set to the lowest setting. Calibration [Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.] Distance between the smart phone cradle and drop = [Instructions: See worksheet page 6.] Solutions Used for Calibration [Instructions: See worksheet page 6.] Calf Thymus DNA solution concentration (microg/mL) Volume of the 2X DNA solution (μL) Volume of the SYBR GREEN I Dye solution (μL) Final DNA concentration in SYBR Green I Assay (ng/mL) 5 80 80 2.5 2 80 80 1 1 80 80 0.5 0.5 80 80 0.25 0.25 80 80 0.125 0 80 80 blank [Add more rows as needed] Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Analysis With DNA Without DNA Representative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)] Image J Values for All Samples [Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.] TABLE GOES HERE Fitting a Straight Line [Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]