BME100 f2013:W900 Group17 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Abrar Bakhsh Role: Protocol Planning Name: Jeremy Becker Role: Open PCR Machine Testing Name: Luis Hernandez Role: Research and Development Name: Alison Llave Role: Open PCR Machine Testing Name: Naaz Maududi Role: Research and Development LAB 1 WRITE-UP Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run The PCR machine was first used to run through a test cycle on October 23, 2013 at 9:56 am and stopped at 11:16 am for a total run of one hour and twenty minutes. During this time the machine ran without any major problems and in fact was very quiet throughout the cycles it went through. Also, the time to completion displayed on the computer monitor through the program was always within reasonable parameters given the average time it takes for the PCR machine to complete its ideal number of cycles. Given the time that the PCR machine in this experiment was allowed to operate, one hour and twenty minutes, it was able to complete 25 cycles in total with thirty six minutes being left of operation time as inficated by the timer. Protocols Thermal Cycler Program DNA Sample Set-up row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... PCR Reaction Mix What is in the PCR reaction mix? DNA/ primer mix What is in the DNA/ primer mix? Research and Development PCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) Cancerous DNA will undergo an exponential growth that is shown in the images http://universe-review.ca/I11-50-PCR.jpg http://www.nature.com/scitable/nated/content/19492/pierce_18_19_large_2.jpg https://www.google.com/searchq=process+of+pcr&source=lnms&tbm=isch&sa=X&ei=yFRwUqGTOIKxiwKY64CADw&sqi=2&ved=0CAcQ_AUoAQ&biw=1951&bih=961#q=process+of+pcr+amplification&tbm=isch&facrc=_&imgdii=_&imgrc=DFzRQQKNLcbzVM%3A%3BpKI0UaXZHG8oRM%3Bhttp%253A%252F%252Fwww.nature.com%252Fscitable%252Fnated%252Fcontent%252F19492%252Fpierce_18_19_large_2.jpg%3Bhttp%253A%252F%252Fwww.nature.com%252Fscitable%252Ftopicpage%252Fthe-biotechnology-revolution-pcr-and-the-use-553%3B700%3B323