BME100 f2013:W900 Group18 L4: Difference between revisions
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| [[Image:DanielM.jpg|250px|thumb|Name: Daniel McDermand]] | | [[Image:DanielM.jpg|250px|thumb|Name: Daniel McDermand]] | ||
| [[Image: | | [[Image:Photoofdylan.jpg|100px|thumb|Name: Dylan DeBruin]] | ||
| [[Image:Photo on 10-29-13 at 7.48 PM -3.jpg |100px|thumb|Name: Nick Vale]] | | [[Image:Photo on 10-29-13 at 7.48 PM -3.jpg |100px|thumb|Name: Nick Vale]] | ||
| [[Image:kir.jpg|100px|thumb|Name: Kirstin Peters]] | | [[Image:kir.jpg|100px|thumb|Name: Kirstin Peters]] | ||
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'''The Original Design'''<br> | '''The Original Design'''<br> | ||
[[Image:OpenPCR Machine side view2.jpg]] <br> | |||
Displayed above, the OpenPCR Machine is used to amplify DNA in a short amount of time. PCR, or polymerase chain reaction, is a process that essentially replicates a small amount of DNA until there is a substantial amount present for testing. The DNA in the machine is subject to dynamic environment changes, as the machine rapidly heats and cools the single stranded sample until multiple copies of the original DNA are created. The OpenPCR Machine is connected to a computer via a USB cable, which allows the replication process to be controlled entirely on the computer screen. In order to run an experiment, temperatures and a desired amount of cycles must be entered on the computer, info which is then transmitted to the machine in order to run the process. After the inputted information is received by the OpenPCR Machine, the DNA inside will be subject to the exact amount of cycles and temperature conditions that were set on the computer. The machine will then read results of the completed process which are clearly displayed on an LCD screen on the OpenPCR mechanism itself. <br><br> | Displayed above, the OpenPCR Machine is used to amplify DNA in a short amount of time. PCR, or polymerase chain reaction, is a process that essentially replicates a small amount of DNA until there is a substantial amount present for testing. The DNA in the machine is subject to dynamic environment changes, as the machine rapidly heats and cools the single stranded sample until multiple copies of the original DNA are created. The OpenPCR Machine is connected to a computer via a USB cable, which allows the replication process to be controlled entirely on the computer screen. In order to run an experiment, temperatures and a desired amount of cycles must be entered on the computer, info which is then transmitted to the machine in order to run the process. After the inputted information is received by the OpenPCR Machine, the DNA inside will be subject to the exact amount of cycles and temperature conditions that were set on the computer. The machine will then read results of the completed process which are clearly displayed on an LCD screen on the OpenPCR mechanism itself. <br><br> | ||
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'''Base-Pairing'''<br> | '''Base-Pairing'''<br> | ||
There are four different bases, or nucleotides, that compose a DNA strand. The four bases are as follows: Adenine (A), Thymine (T), Cytosine (C), and Guanine (G). These nucleotides are bonded to their complimentary base on the template DNA strand by hydrogen bonding to create a double-helix DNA strand. The nucleotides can only attach to a different specified nucleotide; adenine bonds to thymine (and vice versa), and cytosine bonds to guanine (and vice versa).<br> | There are four different bases, or nucleotides, that compose a DNA strand. The four bases are as follows: Adenine (A), Thymine (T), Cytosine (C), and Guanine (G). These nucleotides are bonded to their complimentary base on the template DNA strand by hydrogen bonding to create a double-helix DNA strand. The nucleotides can only attach to a different specified nucleotide; adenine bonds to thymine (and vice versa), and cytosine bonds to guanine (and vice versa).<br><br> | ||
'''Primers on Cancer DNA'''<br> | |||
[[Image:PrimerNick.png]]<br> | |||
Source: http://learn.genetics.utah.edu/content/labs/pcr/ <br><br> | |||
'''Polymerase Sythesizing''' <br> | |||
[[Image:Polymerase.png]]<br> | |||
Source: http://learn.genetics.utah.edu/content/labs/pcr/ | |||
Latest revision as of 16:40, 3 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine failed to display any information on the LCD projector. This is evidence that part 3 and part 6 connect the circuit board to the LCD display, so when unplugged, the machine was unable to display any results. When we unplugged the white wire that connects (part 6) to (part 2), the machine displayed a temperature that was lower than the actual temperature inside the system. This is evidence that part 2 and part 6 connect the circuit board to the temperature pad, so when unplugged, the PCR machine failed to display accurate temperature readings.
The first test run of OpenPCR Machine 1 was conducted at 10:00 a.m. on October 23, 2013. The machine ran silently for two hours, and no complications were present. Data displayed on the LCD screen corresponded with data displayed on the computer software program, and the data displayed a steady increase in temperature as time went by. This is because the DNA needed to be raised to a temperature of 95 degrees Celsius to begin the process of copying. However, even though our machine displayed data that was consistent on both the computer and the machine itself, our team realized that a substantial amount of time is needed to run multiple cycles and produce enough duplicates of the targeted section, as each cycle takes around three to four minutes to complete.
ProtocolsThermal Cycler Program
DNA Sample Set-up Procedure
1. Gather the sixteen mixes.
Research and DevelopmentPCR - The Underlying Technology Component Function of a PCR Reaction Steps of Thermal Cycling Base-Pairing Primers on Cancer DNA Polymerase Sythesizing
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