BME100 f2013:W900 Group9 L5: Difference between revisions
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* Type of Smartphone: iPhone 5 | * Type of Smartphone: iPhone 5 | ||
** Flash: off | ** Flash: off | ||
** ISO setting: auto ( | ** ISO setting: auto (no option on model used) | ||
** White Balance: auto ( | ** White Balance: auto (no option on model used) | ||
** Exposure: auto ( | ** Exposure: auto (no option on model used) | ||
** Saturation: auto ( | ** Saturation: auto (no option on model used) | ||
** Contrast: auto ( | ** Contrast: auto (no option on model used) | ||
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'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
# | # Attach fresh tip to micropipette and set micropipette to 50 micro-liters | ||
# | # Push micropipette plunger to first stop, and place tip into SYBR Green 1 dye, release plunger | ||
# | # Lift tip out of SYBR Green 1 dye and hover micropipette tip directly between two holes of the fluorimeter slide | ||
# | # Push plunger down to second stop, allowing dye to release onto the slide | ||
# | # Repeat steps 1-4 but now with the DNA | ||
# | # After observing the solution on the fluorimeter, discard the solution | ||
# | # Push micropipette plunger to first stop, place tip in the solution on the slide, and release plunger | ||
# | # Repeat step 7 if solution still remains on slide, discard micropipette tip | ||
# | # Repeat steps 1-8 for remaining DNA, make sure to use each hole on the slide only once | ||
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Latest revision as of 23:44, 12 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye Single-Drop Fluorimeter How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
Solutions Used for Calibration
Data AnalysisRepresentative Images of Samples 0.0 μg/mL DNA Concentration Image J Analysis
Image J Values for All Samples
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