BYU iGEM/Notebook/11 February 2011: Difference between revisions
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<b>ara-C pBAD Reverse Primer:</b> | <b>ara-C pBAD Reverse Primer:</b> | ||
cggCTGCAGaaaaacgggTatggagaaaca | cggCTGCAGaaaaacgggTatggagaaaca | ||
==Oxy Team== | |||
Redesigned the multiple cloning site (MCS) map in light of some new information regarding the OxyR binding site. See image below. | |||
[[Image:IMAG0070.jpg]] | [[Image:IMAG0070.jpg]] | ||
Revision as of 14:42, 11 February 2011
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Thermoswitch Team
We've designed our forward and reverse primers to clone out the ara-C gene and pBAD promoter for our thermoswitch plasmid.
ara-C pBAD Forward Primer:
gccAGATCTtaccgggtagaatcaaaccga
ara-C pBAD Reverse Primer: cggCTGCAGaaaaacgggTatggagaaaca
Oxy Team
Redesigned the multiple cloning site (MCS) map in light of some new information regarding the OxyR binding site. See image below.
