BioMicroCenter:SPRI-Works: Difference between revisions
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[[Image: BioMicro_SPRIworks.jpg|right|200px]] | [[Image: BioMicro_SPRIworks.jpg|right|200px]] | ||
The | The SPRI-te Nucleic Acid Extractor is a fully-automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. It performs all of the traditional Illumina protocol steps listed on the [[BioMicroCenter:Illumina Library Preparation|Illumina Library Preparation page]] except for PCR enrichment. A total of 10 libraries may be prepared simultaneously in 5 hours. | ||
==How it Works== | ==How it Works== | ||
[[Image:spri-works.jpg|right|200px]] | [[Image:spri-works.jpg|right|200px]] | ||
The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that | The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that contains the liquid handling program, and a cartridge containing all of the reagents required to prepare a single library. | ||
The platform utilizes AMPureXP DNA-binding magnetic beads to purify fragmented DNA and perform size selection, eliminating the need for column purification and gel-based size selection. There are three size range options available, 200-400bp, 300-600bp, or no size selection.<br> | |||
After the adapter ligation and size selection are complete, samples are ready for PCR enrichment and clean-up to complete the library prep process. | |||
For more information on the SPRI-Works system please visit [http://spriworks.com www.spriworks.com]. | For more information on the SPRI-Works system please visit [http://spriworks.com www.spriworks.com]. | ||
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Sample concentrations for the SPRI-TE robot are highly variable, we have successfully run samples as low as 0.3ng/uL. It is suggested that you submit as much volume as possible, as the first step of the protocol is to bring all sample volumes up to 100uLs in water. | Sample concentrations for the SPRI-TE robot are highly variable, we have successfully run samples as low as 0.3ng/uL. It is suggested that you submit as much volume as possible, as the first step of the protocol is to bring all sample volumes up to 100uLs in water. | ||
All samples submitted for direct SPRI-Works library preparation must be submitted with '''user supplied''' adapter mix. Adapter mixes submitted should be previously diluted and should have enough volume for 10uLs of adapter per sample. For recommended dilutions for various sample concentrations please see the chart below: | All samples submitted for direct SPRI-Works library preparation (SPRI-only) must be submitted with '''user supplied''' adapter mix. Adapter mixes submitted should be previously diluted and should have enough volume for 10uLs of adapter per sample. For recommended dilutions for various sample concentrations please see the chart below: | ||
[[Image:Spri-adaptrecomend.jpg|center|500px]] | [[Image:Spri-adaptrecomend.jpg|center|500px]] | ||
Revision as of 12:51, 28 September 2011
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The SPRI-te Nucleic Acid Extractor is a fully-automated library preparation system for Illumina sequencing and supports all Illumina applications except for smallRNA-Seq. It performs all of the traditional Illumina protocol steps listed on the Illumina Library Preparation page except for PCR enrichment. A total of 10 libraries may be prepared simultaneously in 5 hours.
How it Works
The SPRI-works system consists of three main components: the SPRI-TE Nucleic Acid Extractor, a method card that contains the liquid handling program, and a cartridge containing all of the reagents required to prepare a single library.
The platform utilizes AMPureXP DNA-binding magnetic beads to purify fragmented DNA and perform size selection, eliminating the need for column purification and gel-based size selection. There are three size range options available, 200-400bp, 300-600bp, or no size selection.
After the adapter ligation and size selection are complete, samples are ready for PCR enrichment and clean-up to complete the library prep process.
For more information on the SPRI-Works system please visit www.spriworks.com.
Sample Submission
Sample concentrations for the SPRI-TE robot are highly variable, we have successfully run samples as low as 0.3ng/uL. It is suggested that you submit as much volume as possible, as the first step of the protocol is to bring all sample volumes up to 100uLs in water.
All samples submitted for direct SPRI-Works library preparation (SPRI-only) must be submitted with user supplied adapter mix. Adapter mixes submitted should be previously diluted and should have enough volume for 10uLs of adapter per sample. For recommended dilutions for various sample concentrations please see the chart below:
Please note that samples will need to be resubmitted separately for Illumina sequencing.
