Biomolecular Breadboards:Protocols: Difference between revisions
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{| border="1" cellpadding="5" style="width: 800px" | {| border="1" cellpadding="5" style="width: 800px" | ||
! scope="col" | Component | ! scope="col" | Component | ||
! scope="col" | Material price per μL of reaction | ! scope="col" | Material price per | ||
! scope="col" | Labor price per μL of reaction | μL of reaction | ||
! scope="col" | Total price per μL of reaction | ! scope="col" | Labor price per | ||
! scope="col" | Total price per 12 μL reaction | μL of reaction | ||
! scope="col" | Total price per | |||
μL of reaction | |||
! scope="col" | Total price per | |||
12 μL reaction | |||
|- | |- | ||
| style="width: | | style="width: 150px;" | ''E. coli'' crude extract || style="text-align:center; width: 100px;" | 0.45 cents || style="text-align:center; width: 100px;" | 0.62 cents || style="text-align:center; width: 100px;" | 1.07 cents || style="text-align:center; width: 100px;" | 12.84 cents | ||
|- | |- | ||
| Amino acids || style="text-align:center" | 0.21 cents || style="text-align:center" | 0. | | Amino acids || style="text-align:center" | 0.21 cents || style="text-align:center" | 0.03 cents || style="text-align:center" | 0.24 cents || style="text-align:center" | 2.93 cents | ||
|- | |- | ||
| 3-PGA || style="text-align:center" | 0.39 cents || style="text-align:center" | 0.94 cents || style="text-align:center" | 1.33 cents || style="text-align:center" | 15.96 cents | | 3-PGA || style="text-align:center" | 0.39 cents || style="text-align:center" | 0.94 cents || style="text-align:center" | 1.33 cents || style="text-align:center" | 15.96 cents | ||
Revision as of 23:54, 12 August 2012
| Home | Protocols | DNA parts | Preliminary Data | Models | More Info |
Cell-free circuit breadboard
The following protocols are available for the cell-free circuit breadboard:
- Reaction Preparation - Cell-free expression of circuits from linear or plasmid DNA
Cost estimate
The cell-free circuit breadboard costs approximately 2.64 cents per uL, or 31.7 cents per 12 uL reaction. The cost distribution is shown in the table below.
| Component | Material price per
μL of reaction |
Labor price per
μL of reaction |
Total price per
μL of reaction |
Total price per
12 μL reaction |
|---|---|---|---|---|
| E. coli crude extract | 0.45 cents | 0.62 cents | 1.07 cents | 12.84 cents |
| Amino acids | 0.21 cents | 0.03 cents | 0.24 cents | 2.93 cents |
| 3-PGA | 0.39 cents | 0.94 cents | 1.33 cents | 15.96 cents |
| Total price | 1.05 cents | 1.6 cents | 2.64 cents | 31.7 cents |
For a more detailed cost estimate, please see Breadboard Cost Estimate.
