Biomolecular Breadboards:Protocols

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{| border="1" cellpadding="5" style="width: 800px"
! scope="col" | Component
! scope="col" | Component
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! scope="col" | Material price per μL of reaction
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! scope="col" | Material price per  
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! scope="col" | Labor price per μL of reaction
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μL of reaction
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! scope="col" | Total price per μL of reaction
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! scope="col" | Labor price per  
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! scope="col" | Total price per 12 μL reaction
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μL of reaction
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! scope="col" | Total price per  
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μL of reaction
 +
! scope="col" | Total price per  
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12 μL reaction
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| style="width: 200px;" | ''E. coli'' crude extract || style="text-align:center; width: 100px;" | 0.45 cents || style="text-align:center; width: 100px;" | 0.62 cents || style="text-align:center; width: 100px;" | 1.07 cents || style="text-align:center; width: 100px;" | 12.84 cents
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| style="width: 150px;" | ''E. coli'' crude extract || style="text-align:center; width: 100px;" | 0.45 cents || style="text-align:center; width: 100px;" | 0.62 cents || style="text-align:center; width: 100px;" | 1.07 cents || style="text-align:center; width: 100px;" | 12.84 cents
|-
|-
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| Amino acids ||  style="text-align:center" | 0.21 cents || style="text-align:center" | 0.034 cents || style="text-align:center" | 0.244 cents || style="text-align:center" | 2.93 cents
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| Amino acids ||  style="text-align:center" | 0.21 cents || style="text-align:center" | 0.03 cents || style="text-align:center" | 0.24 cents || style="text-align:center" | 2.93 cents
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| 3-PGA ||  style="text-align:center" | 0.39 cents || style="text-align:center" | 0.94 cents || style="text-align:center" | 1.33 cents || style="text-align:center" | 15.96 cents
| 3-PGA ||  style="text-align:center" | 0.39 cents || style="text-align:center" | 0.94 cents || style="text-align:center" | 1.33 cents || style="text-align:center" | 15.96 cents

Revision as of 02:54, 13 August 2012

Home Protocols DNA parts Preliminary Data Models More Info

Cell-free circuit breadboard

The following protocols are available for the cell-free circuit breadboard:


Cost estimate

The cell-free circuit breadboard costs approximately 2.64 cents per uL, or 31.7 cents per 12 uL reaction. The cost distribution is shown in the table below.

Component Material price per

μL of reaction

Labor price per

μL of reaction

Total price per

μL of reaction

Total price per

12 μL reaction

E. coli crude extract 0.45 cents 0.62 cents 1.07 cents 12.84 cents
Amino acids 0.21 cents 0.03 cents 0.24 cents 2.93 cents
3-PGA 0.39 cents 0.94 cents 1.33 cents 15.96 cents
Total price 1.05 cents 1.6 cents 2.64 cents 31.7 cents

For a more detailed cost estimate, please see Breadboard Cost Estimate.

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