Biomolecular Breadboards:Protocols:3-PGA Buffer Prep: Difference between revisions
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| 3-PGA || 1.4M || 30mM || 128.6µl || 514.4µl || 771.6µl | | 3-PGA || 1.4M || 30mM || 128.6µl || 514.4µl || 771.6µl | ||
|} | |} | ||
{| border="1" cellpadding="2" style="width: | {| border="1" cellpadding="2" style="width: 580px" | ||
| style="width: | | style="width: 365px;" | Total Volume || style="width: 70px;" | 427µl || style="width: 70px;" | 1708µl || style="width: 70px;" | 2562µl | ||
|} | |} |
Revision as of 23:17, 5 June 2012
Home | Protocols | DNA parts | Preliminary Data | Models | More Info |
Preparation of 3-PGA Buffer for the Cell-Free Expression System, 14X
Component | Stock | Final Concentration in cell-free reaction | 1-fold | 4-fold | 6-fold |
---|---|---|---|---|---|
HEPES pH8 | 2M | 50mM | 150µl | 600µl | 900µl |
Water | 6µl | 24µl | 36µl | ||
Homemade Nucleotides | 156mM: ATP & GTP 94mM: CTP & UTP | 1.5mM: ATP & GTP 0.9mM: CTP & UTP | 57.8µl | 231.2µl | 346.8µl |
tRNA E.coli | 50mg/ml | 0.2mg/ml | 24µl | 96µl | 144µl |
CoA | 65mM | 0.26mM | 24µl | 96µl | 144µl |
NAD | 175mM | 0.33mM | 11.5µl | 46µl | 69µl |
cAMP | 650mM | 0.75mM | 7.1µl | 28.4µl | 42.6µl |
Folinic Acid | 33.9mM | 0.068mM | 12µl | 48µl | 72µl |
Spermidine | 1M | 1mM | 6µl | 24µl | 36µl |
3-PGA | 1.4M | 30mM | 128.6µl | 514.4µl | 771.6µl |
Total Volume | 427µl | 1708µl | 2562µl |