Buckner Lab:Notebook/Summer 2010/2010/05/19: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">DNA Purification and Gel Electrophoresis</span> | ||
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== | ==Plasmid Isolation== | ||
* | * Used QIAprep isolation kit from Qiagen to isolate plasmids. | ||
* Performed EcoR1 digestion to isolated DNA for ~2.25 hrs | |||
==Pouring and Running Gel== | |||
* Made a 0.8% agarose gel with ethidium bromide | |||
* Ran the gel for 1.5 hrs in TAE buffer at 60V. | |||
* Photographed and annotated gels | |||
-Didn't allow the gel to run long enough. <br> | |||
-Need to add all of the DNA from the prep tube when taking a "look" after an EcoRI digestion | |||
Revision as of 11:55, 21 May 2010
 DNA Purification and Gel Electrophoresis
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Plasmid Isolation
Pouring and Running Gel
-Didn't allow the gel to run long enough.
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