Erickson:TAE Buffer: Difference between revisions

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(New page: [http://openwetware.org/wiki/Erickson:Protocols Back to Protocols]<br><br><br> '''TAE Running Buffer''' <br><br> ''50X TAE Buffer'' ''Tris-Acetate-EDTA''<br> 242 mg Tris Base 57....)
 
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[http://openwetware.org/wiki/Erickson:Protocols Back to Protocols]<br><br><br>
{{Template:Erickson lab}}


'''TAE Running Buffer'''
'''TAE Running Buffer'''
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''50X TAE Buffer''
''50X TAE Buffer''
   ''Tris-Acetate-EDTA''<br>
   ''Tris-Acetate-EDTA''<br>
   242 mg Tris Base
   242 g Tris Base
   57.1 mL Acetic Acid
   57.1 mL Acetic Acid
   100 mL  0.5M EDTA
   100 mL  0.5M EDTA


*Add ddH2O to 1 L and adjust pH to 8.5
*Add ddH<sub>2</sub>O to 1 L and adjust pH to 8.5

Latest revision as of 15:28, 30 November 2009


TAE Running Buffer

50X TAE Buffer

  Tris-Acetate-EDTA
242 g Tris Base 57.1 mL Acetic Acid 100 mL 0.5M EDTA
  • Add ddH2O to 1 L and adjust pH to 8.5