User contributions for Bria Walch
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30 November 2009
- 15:2815:28, 30 November 2009 diff hist −1 Erickson:TAE Buffer No edit summary current
16 November 2009
- 17:2617:26, 16 November 2009 diff hist −1 Erickson:Lab Members No edit summary
10 November 2009
- 12:0312:03, 10 November 2009 diff hist +15 Erickson:2-log Ladder No edit summary current
- 12:0112:01, 10 November 2009 diff hist +44 N Erickson:2-log Ladder New page: 1 part 2-log ladder 1 part dye 4 parts water
- 12:0012:00, 10 November 2009 diff hist +40 Erickson:Protocols →PCR current
26 August 2009
- 12:4912:49, 26 August 2009 diff hist −1 User:Bria Walch No edit summary current
1 July 2009
- 15:5315:53, 1 July 2009 diff hist +1 Erickson:Flea Crushing No edit summary current
- 15:5215:52, 1 July 2009 diff hist −15 Erickson:Flea Crushing No edit summary
- 15:5215:52, 1 July 2009 diff hist +15 Erickson:Flea Crushing No edit summary
- 15:5015:50, 1 July 2009 diff hist +1,589 N Erickson:Flea Crushing New page: Flea Crushing Beforehand, gather infected fleas into an microcentrifuge tube and place in -80 freezer to kill fleas. Remove fleas from freezer. Sterilize fleas with two was...
- 15:3215:32, 1 July 2009 diff hist +46 Erickson:Protocols No edit summary
29 June 2009
- 16:1716:17, 29 June 2009 diff hist +441 N Erickson:Frozen Stocks New page: Frozen Stocks Prepare an overnight culture of your bacteria using the appropriate antibiotics. Subculture the bacteria the next morning. When the culture is thick and cloud... current
- 16:1216:12, 29 June 2009 diff hist +46 Erickson:Protocols No edit summary
27 May 2009
- 11:1011:10, 27 May 2009 diff hist +100 Erickson:TB No edit summary current
- 09:5009:50, 27 May 2009 diff hist 0 Erickson:PCR Clean-up for Sequencing No edit summary current
26 May 2009
- 11:3611:36, 26 May 2009 diff hist +1 Erickson:PCR Clean-up for Sequencing No edit summary
- 11:3611:36, 26 May 2009 diff hist +1,121 N Erickson:PCR Clean-up for Sequencing New page: PCR Clean-up for Sequencing To remove excess dNTPs and unincorporated primers, use the Exo-SAP method below. Materials: PCR product Exo-SAP (recipe for 100 uL, enough to cl...
- 11:2811:28, 26 May 2009 diff hist +74 Erickson:Protocols No edit summary
14 May 2009
- 16:3416:34, 14 May 2009 diff hist +27 Erickson:TB No edit summary
- 16:3316:33, 14 May 2009 diff hist +38 Erickson:TB No edit summary
- 16:3116:31, 14 May 2009 diff hist +57 N Erickson:TB New page: TB Media 27.5 g TB 500 mL water 7.5 g agar
- 16:2416:24, 14 May 2009 diff hist +3 Erickson:Protocols No edit summary
- 16:2316:23, 14 May 2009 diff hist +21 Erickson:Protocols No edit summary
- 16:0516:05, 14 May 2009 diff hist +1 Erickson:Flea No edit summary current
29 April 2009
- 15:2015:20, 29 April 2009 diff hist +70 Erickson:Kanamycin No edit summary current
- 15:1815:18, 29 April 2009 diff hist +1 Erickson:Irgasan No edit summary current
- 15:1715:17, 29 April 2009 diff hist +61 Erickson:Irgasan No edit summary
27 April 2009
- 11:0811:08, 27 April 2009 diff hist +59 Erickson:RNA No edit summary current
- 11:0611:06, 27 April 2009 diff hist +214 Erickson:RNA No edit summary
24 April 2009
- 15:4415:44, 24 April 2009 diff hist +11 Erickson:Tri-parental Mating No edit summary current
- 15:4315:43, 24 April 2009 diff hist +33 Erickson: Electroporation No edit summary current
- 15:4215:42, 24 April 2009 diff hist +56 Erickson: Electroporation No edit summary
- 15:4015:40, 24 April 2009 diff hist +2 Erickson: Electroporation No edit summary
- 11:0611:06, 24 April 2009 diff hist +13 Erickson: Electroporation No edit summary
2 July 2008
- 18:0018:00, 2 July 2008 diff hist −11 Erickson:Chloramphenicol No edit summary current
- 12:4812:48, 2 July 2008 diff hist +50 Erickson:Flea No edit summary
23 June 2008
- 13:3313:33, 23 June 2008 diff hist −17 Erickson:Irgasan No edit summary
- 11:3611:36, 23 June 2008 diff hist +10 Erickson:Tri-parental Mating No edit summary
- 11:3511:35, 23 June 2008 diff hist +21 Erickson: Electroporation No edit summary
- 11:3411:34, 23 June 2008 diff hist +6 Erickson: Bi-parental Mating No edit summary current
- 11:3211:32, 23 June 2008 diff hist +817 N Erickson: Bi-parental Mating New page: 1. Grow up the separate cultures in the selective media overnight: BO81 in BHI + Strep B628 in BHI + Km + Cm B629 in BHI + Km + Cm Y. pestis KIM6+ in BHI + Irg Y. ...
- 11:1911:19, 23 June 2008 diff hist +58 Erickson:Protocols →Transposon Mutagenesis
2 June 2008
- 09:5809:58, 2 June 2008 diff hist +578 Erickson: Electroporation No edit summary
- 09:5709:57, 2 June 2008 diff hist +883 N Erickson: Electroporation New page: Transformation by Electroporation Competent cells Prepare 5 mL of bacterial strain that you wish to transform in BHI. Grow overnight . Chill sterile microfuge tubes on ice and turn on the ...
- 09:5509:55, 2 June 2008 diff hist +53 Erickson:Protocols →Bacterial Conjugation
- 09:5409:54, 2 June 2008 diff hist −6 User:Bria Walch No edit summary
20 May 2008
- 17:0117:01, 20 May 2008 diff hist +595 N Erickson:Tri-parental Mating New page: Tri-parental Mating 1. Grow the donor, helper, and recipient strains of bacteria separately in 3 mL of LB Broth overnight (30° C for Y. pestis and 37°C for E. coli) 2. Combine 200 µL of...
- 17:0017:00, 20 May 2008 diff hist 0 Erickson:Protocols No edit summary
- 17:0017:00, 20 May 2008 diff hist +84 Erickson:Protocols No edit summary
- 16:5516:55, 20 May 2008 diff hist 0 User:Bria Walch No edit summary
