Gaussian Fitting for Resolution
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Scholars,
Two Matlab functions – gaussfit and plotgaussfit – will be of extreme utility in estimating the resolution of your microscope from the PSD slide. The m-files for these functions have been placed in the fluorescence microscopy lab folder. Documentation is available at: [1].
The best approach might be to fit several objects and take an average. Please report the uncertainty in your estimate.
--Steve
Many thanks to David Kolin, the author of this excellent code.