Gaussian Fitting for Resolution
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Two Matlab functions – gaussfit and plotgaussfit – will be of extreme utility in estimating the resolution of your microscope from the PSD slide. The m-files for these functions have been placed in the fluorescence microscopy lab folder. Documentation is available at: .
The best approach might be to fit several objects and take an average. Please report the uncertainty in your estimate.
Many thanks to David Kolin, the author of this excellent code.