Generating antibodies

Drawing of an antibody - heavy chain red, light chain yellow.

You are hot in pursuit of an interesting protein but there are no commercial antibodies or they are bad. It's time to think about raising your own antibodies against the protein. It will be a time and money consuming undertaking but once you developed a specific antibody or set of antibodies, you will be in a unique position to generate interesting data.

See also

• Protein purification tags
• Western blot

External links

• Introduction to monoclonal antibody generation by Mark Soloski, Johns Hopkins
• FAQ on antibody generation by Alta Bioscience, UK
• Hybridoma and in vitro generation of antibodies, Antibody Facility, UNC Chapel Hill
• Monoclonal Antibodies: A Practical Approach by Philip Shepherd, Christopher Dean on Google Book Search
• Protocol for raising polyclonal antibodies in rabbits, Chew lab, Uni of Singapore

Protocol Online (methods forum)

• Protocol-online.org archive, keyword antibody and discussions indexed under antibody
• Discussion on affinity purification
• Discussion on antigen amount

Wikipedia

• Antibody, esp. research applications
• Polyclonal antibodies, Monoclonal antibodies