Gill:Protocols: Difference between revisions
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===Microbiology and Molecular Biology=== | ===Microbiology and Molecular Biology=== | ||
[[Gill:Calcium chloride competent cells |Calcium chloride competent cells]] | |||
[[Gill:Chung cells |Chung cells]] | [[Gill:Chung cells |Chung cells]] | ||
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[[Gill:Preparing phage specimens for TEM |Preparing phage specimens for TEM]] | [[Gill:Preparing phage specimens for TEM |Preparing phage specimens for TEM]] | ||
[[Gill:Rapid bacterial DNA prep |Rapid bacterial DNA prep]] | |||
[[Gill:S. aureus genomic DNA isolation |S. aureus genomic DNA isolation]] | [[Gill:S. aureus genomic DNA isolation |S. aureus genomic DNA isolation]] | ||
[[Gill:S. aureus pre-treatment for plasmid preps |S. aureus pre-treatment for plasmid preps]] | |||
[[Gill:Spot complementation test |Spot complementation test]] | |||
[[Gill:Subculturing phage isolates |Subculturing phage isolates]] | [[Gill:Subculturing phage isolates |Subculturing phage isolates]] |
Revision as of 12:55, 30 April 2015
Protocols
Microbiology and Molecular Biology
Calcium chloride competent cells
Confirming phage via restriction digest and gel electrophoresis
Preparing phage specimens for TEM
S. aureus genomic DNA isolation
S. aureus pre-treatment for plasmid preps
Solutions and Media Preparation
10x Lambda diluent (no gelatin)