Gill:M9 agar

Materials

	500 mL	1000 mL
Bacto Agar	7.5 g	15 g
dH2O	450 ml	880 ml
10 X M9 salts + glucose	50 ml	100 ml
1 M MgSO4	1 ml	2 ml	(2 mM final)
1 M CaCl2	50 µl	0.1 ml	(0.1 mM final)

Procedure

Autoclave agar/water solution, let cool. Aseptically add sterile salts + glucose, pour.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!