Haynes Lab:Notebook/CRISPR Editing/2014/07/06

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(Autocreate 2014/07/06 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
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==Entry title==
==Entry title==
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* Insert content here...
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Results from yesterday's ligation <br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Backbone'''
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| align="center" style="background:#f0f0f0;"|'''Insert'''
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| align="center" style="background:#f0f0f0;"|'''# colonies'''
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|-
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| pX330||bb ctrl||13
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|-
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| pX330||g14||30
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|-
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| pX330||g5||20
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|-
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| pX330||g13||29
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|-
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| pX330||gn1T||21
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|-
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| pX330||gn1B||8
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|-
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| pX330||gn3T||22
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|-
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| pX330||gnBb B||20
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|-
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| pX335||bb ctrl||19
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|-
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| pX335||gn3T||14
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|-
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| pX335||gn1B||19
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|-
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| pX335||gn1T||23
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|-
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| pX335||gn3bB||23
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|}
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<br><br>
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Zhang lab help pages say overnight ligations can lead to many colonies on the backbone plate. Picked 6 colonies from plate pX330-g14 plate.  

Revision as of 16:13, 8 July 2014

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Entry title

Results from yesterday's ligation

Backbone Insert # colonies
pX330bb ctrl13
pX330g1430
pX330g520
pX330g1329
pX330gn1T21
pX330gn1B8
pX330gn3T22
pX330gnBb B20
pX335bb ctrl19
pX335gn3T14
pX335gn1B19
pX335gn1T23
pX335gn3bB23



Zhang lab help pages say overnight ligations can lead to many colonies on the backbone plate. Picked 6 colonies from plate pX330-g14 plate.



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