Haynes Lab:Notebook/CRISPR Editing/2014/09/25: Difference between revisions
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Annealing protocol on wang lab machine | Annealing protocol on wang lab machine | ||
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Digest with surveyor, follow same protocol from yesterday, 0.5ul nuclease for 45mins at 42deg | Digest with surveyor, follow same protocol from yesterday, 0.5ul nuclease for 45mins at 42deg<br>Added 1ul stop solution, kept on ice for about 5 minutes, ran on gel with the same amount of PCR product in the next lane. | ||
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Ran the nested PCR reactions on gel | |||
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Revision as of 11:52, 25 September 2014
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09/25/2014Purified PCR product for Surveyor, P197,P198 forgot to save some unpurified on a gel
Set up nested PCR with P199 and P200 of gRNA 20 samples and gRNA 29 samples and Luc14 genomic DNA (7 50ul reactions in triplicate to get enough)
Annealing protocol on wang lab machine
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