Haynes Lab:Notebook/CRISPR Editing/2014/10/22: Difference between revisions

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| align="center" style="background:#f0f0f0;"|'''Distance from gRNA'''
| align="center" style="background:#f0f0f0;"|'''Distance from gRNA'''
|-
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| Luc14 gRNA 20 Srvyr PCR||P197||SRVYR Luc F2||F||390
|1|| Luc14 gRNA 20 Srvyr PCR||P197||SRVYR Luc F2||F||390
|-
|-
| Luc14 gRNA 20 Srvyr PCR||P160||gRNA 029 ES P R||R||238
|2|| Luc14 gRNA 20 Srvyr PCR||P160||gRNA 029 ES P R||R||238
|-
|-
| Gal4-EED +dox 1 gRNA 20 Srvyr PCR||P197||SRVYR Luc F2||F||390
|3|| Gal4-EED +dox 1 gRNA 20 Srvyr PCR||P197||SRVYR Luc F2||F||390
|-
|-
| Gal4-EED +dox 2 gRNA 20 Srvyr PCR||P160||gRNA 029 ES P R||R||238
|4|| Gal4-EED +dox 2 gRNA 20 Srvyr PCR||P160||gRNA 029 ES P R||R||238
|-
|-
| Gal4-EED +dox 1 gRNA 20 Srvyr PCR||P197||SRVYR Luc F2||F||390
|5|| Gal4-EED +dox 1 gRNA 20 Srvyr PCR||P197||SRVYR Luc F2||F||390
|-
|-
| Gal4-EED +dox 2 gRNA 20 Srvyr PCR||P160||gRNA 029 ES P R||R||238
|6|| Gal4-EED +dox 2 gRNA 20 Srvyr PCR||P160||gRNA 029 ES P R||R||238
|-
|-
| Luc14 gRNA 21 Srvyr PCR||P197||SRVYR Luc F2||F||259
|7|| Luc14 gRNA 21 Srvyr PCR||P197||SRVYR Luc F2||F||259
|-
|-
| Luc14 gRNA 22 Srvyr PCR||P197||SRVYR Luc F2||F||341
|8|| Luc14 gRNA 22 Srvyr PCR||P197||SRVYR Luc F2||F||341
|-
|-
| Luc14 gRNA 22 Srvyr PCR||P160||gRNA 029 ES P R||R||284
|9|| Luc14 gRNA 22 Srvyr PCR||P160||gRNA 029 ES P R||R||284
|-
|-
| Luc14 gRNA 27 Srvyr PCR||P169||gRNA 026 US P F||F||172
|10|| Luc14 gRNA 27 Srvyr PCR||P169||gRNA 026 US P F||F||172
|-
|-
| Luc14 gRNA 27 Srvyr PCR||P198||SRVYR Luc R||R||247
|11|| Luc14 gRNA 27 Srvyr PCR||P198||SRVYR Luc R||R||247
|-
|-
| Luc14 gRNA 29 Srvyr PCR||P149||gRNA 020 ES P F||F||238
|12|| Luc14 gRNA 29 Srvyr PCR||P149||gRNA 020 ES P F||F||238
|-
|-
| Luc14 gRNA 29 Srvyr PCR||P176||gRNA 030 US P R||R||195
|13|| Luc14 gRNA 29 Srvyr PCR||P176||gRNA 030 US P R||R||195
|-
|-
| Gal4-EED +dox 1 gRNA 29 Srvyr PCR||P149||gRNA 020 ES P F||F||238
|14|| Gal4-EED +dox 1 gRNA 29 Srvyr PCR||P149||gRNA 020 ES P F||F||238
|-
|-
| Gal4-EED +dox 2 gRNA 29 Srvyr PCR||P176||gRNA 030 US P R||R||195
|15|| Gal4-EED +dox 2 gRNA 29 Srvyr PCR||P176||gRNA 030 US P R||R||195
|-
|-
| Gal4-EED +dox 1 gRNA 29 Srvyr PCR||P149||gRNA 020 ES P F||F||238
|16|| Gal4-EED +dox 1 gRNA 29 Srvyr PCR||P149||gRNA 020 ES P F||F||238
|-
|-
| Gal4-EED +dox 2 gRNA 29 Srvyr PCR||P176||gRNA 030 US P R||R||195
|17|| Gal4-EED +dox 2 gRNA 29 Srvyr PCR||P176||gRNA 030 US P R||R||195
|-
|-
| Luc14 Srvyr PCR||P197||SRVYR Luc F2||F||
|18|| Luc14 Srvyr PCR||P197||SRVYR Luc F2||F||
|-
|-
| Luc14 Srvyr PCR||P160||gRNA 029 ES P R||R||
|19|| Luc14 Srvyr PCR||P160||gRNA 029 ES P R||R||
|-
|-
| Luc14 Srvyr PCR||P169||gRNA 026 US P F||F||
|20|| Luc14 Srvyr PCR||P169||gRNA 026 US P F||F||
|-
|-
| Luc14 Srvyr PCR||P198||SRVYR Luc R||R||
|21|| Luc14 Srvyr PCR||P198||SRVYR Luc R||R||
|-
|-
| Luc14 Srvyr PCR||P149||gRNA 020 ES P F||F||
|22|| Luc14 Srvyr PCR||P149||gRNA 020 ES P F||F||
|-
|-
| Luc14 Srvyr PCR||P176||gRNA 030 US P R||R||
|23|| Luc14 Srvyr PCR||P176||gRNA 030 US P R||R||
|}
|}


Revision as of 14:11, 22 October 2014

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10/22/2014

Sample Primer ' F/R Distance from gRNA
1 Luc14 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
2 Luc14 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
3 Gal4-EED +dox 1 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
4 Gal4-EED +dox 2 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
5 Gal4-EED +dox 1 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
6 Gal4-EED +dox 2 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
7 Luc14 gRNA 21 Srvyr PCR P197 SRVYR Luc F2 F 259
8 Luc14 gRNA 22 Srvyr PCR P197 SRVYR Luc F2 F 341
9 Luc14 gRNA 22 Srvyr PCR P160 gRNA 029 ES P R R 284
10 Luc14 gRNA 27 Srvyr PCR P169 gRNA 026 US P F F 172
11 Luc14 gRNA 27 Srvyr PCR P198 SRVYR Luc R R 247
12 Luc14 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
13 Luc14 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
14 Gal4-EED +dox 1 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
15 Gal4-EED +dox 2 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
16 Gal4-EED +dox 1 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
17 Gal4-EED +dox 2 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
18 Luc14 Srvyr PCR P197 SRVYR Luc F2 F
19 Luc14 Srvyr PCR P160 gRNA 029 ES P R R
20 Luc14 Srvyr PCR P169 gRNA 026 US P F F
21 Luc14 Srvyr PCR P198 SRVYR Luc R R
22 Luc14 Srvyr PCR P149 gRNA 020 ES P F F
23 Luc14 Srvyr PCR P176 gRNA 030 US P R R



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